Publications

Selected Recent Publication

Tang C, Mo X, Niu Q, Wahafu A, Yang X, Qui M, Ivanov AA, Du Y, Fu H. Hypomorph Mutation-Directed Small-Molecule Protein-Protein Interaction Inducers to Restore Mutant SMAD4-Suppressed TGF-β Signaling. Cell Chem Biol. 2020 Dec 4:S2451-9456(20)30473-6. doi: 10.1016/j.chembiol.2020.11.010. PMID: 33326750.

Liu R, Li D, Sun F, Rampoldi A, Maxwell JT, Wu R, Fischbach P, Castellino SM, Du Y, Fu H, Mandawat A, Xu C. Melphalan induces cardiotoxicity through oxidative stress in cardiomyocytes derived from human induced pluripotent stem cells. Stem Cell Res Ther. 2020 Nov 5;11(1):470. doi: 10.1186/s13287-020-01984-1. PMID: 33153480; PMCID: PMC7643439.

Abdel Aziz MH, Fan Y, Liu L, Moasser MM, Fu H, Jura N, Arkin MR. Expression and purification of active human kinases using Pichia pastoris as a general-purpose host. Protein Expr Purif. 2021 Mar;179:105780. doi: 10.1016/j.pep.2020.105780. Epub 2020 Oct 25. PMID: 33115654.

Lei K, Gu X, Alvarado AG, Du Y, Luo S, Ahn EH, Kang SS, Ji B, Liu X, Mao H, Fu H, Kornblum HI, Jin L, Li H, Ye K. Discovery of a dual inhibitor of NQO1 and GSTP1 for treating glioblastoma. J Hematol Oncol. 2020 Oct 21;13(1):141. doi: 10.1186/s13045-020-00979-y. PMID: 33087132; PMCID: PMC7579906.

Yang X, Amgad M, Cooper LAD, Du Y, Fu H, Ivanov AA. High expression of MKK3 is associated with worse clinical outcomes in African American breast cancer patients. J Transl Med. 2020 Sep 1;18(1):334. doi: 10.1186/s12967-020-02502-w. PMID: 32873298; PMCID: PMC7465409.

Du Y, Li X, Niu Q, Mo X, Qui M, Ma T, Kuo CJ, Fu H. Development of a miniaturized 3D organoid culture platform for ultra-high-throughput screening. J Mol Cell Biol. 2020 Aug 1;12(8):630-643. doi: 10.1093/jmcb/mjaa036. PMID: 32678871; PMCID: PMC7751183.

Singh AM, Zhang L, Avery J, Yin A, Du Y, Wang H, Li Z, Fu H, Yin H, Dalton S. Human beige adipocytes for drug discovery and cell therapy in metabolic diseases. Nat Commun. 2020 Jun 2;11(1):2758. doi: 10.1038/s41467-020-16340-3. PMID: 32488069; PMCID: PMC7265435.

Commander R, Wei C, Sharma A, Mouw JK, Burton LJ, Summerbell E, Mahboubi D, Peterson RJ, Konen J, Zhou W, Du Y, Fu H, Shanmugam M, Marcus AI. Subpopulation targeting of pyruvate dehydrogenase and GLUT1 decouples metabolic heterogeneity during collective cancer cell invasion. Nat Commun. 2020 Mar 24;11(1):1533. doi: 10.1038/s41467-020-15219-7. PMID: 32210228; PMCID: PMC7093428.

Gonzalez-Pecchi V, Kwan AK, Doyle S, Ivanov AA, Du Y, Fu H. NSD3S stabilizes MYC through hindering its interaction with FBXW7. J Mol Cell Biol. 2020 Jul 3;12(6):438-447. doi: 10.1093/jmcb/mjz098. PMID: 31638140; PMCID: PMC7333476.

Ivanov AA. Explore Protein-Protein Interactions for Cancer Target Discovery Using the OncoPPi Portal. Methods Mol Biol. 2020;2074:145-164. doi: 10.1007/978-1-4939-9873-9_12. PMID: 31583637.

Mo X, Tang C, Niu Q, Ma T, Du Y, Fu H. HTiP: High-Throughput Immunomodulator Phenotypic Screening Platform to Reveal IAP Antagonists as Anti-cancer Immune Enhancers. Cell Chem Biol. 2019 Mar 21;26(3):331-339.e3. doi: 10.1016/j.chembiol.2018.11.011. Epub 2019 Jan 10. PMID: 30639259; PMCID:
PMC6501824.

Rusnak L, Tang C, Qi Q, Mo X, Fu H. Large tumor suppressor 2, LATS2, activates JNK in a kinase-independent mechanism through ASK1. J Mol Cell Biol. 2018 Dec 1;10(6):549-558. doi: 10.1093/jmcb/mjy061. PMID: 30496488; PMCID: PMC7191879.

Grzeskowiak CL, Kundu ST, Mo X, Ivanov AA, Zagorodna O, Lu H, Chapple RH, Tsang YH, Moreno D, Mosqueda M, Eterovic K, Fradette JJ, Ahmad S, Chen F, Chong Z, Chen K, Creighton CJ, Fu H, Mills GB, Gibbons DL, Scott KL. In vivo screening identifies GATAD2B as a metastasis driver in KRAS-driven lung cancer. Nat Commun. 2018 Jul 16;9(1):2732. doi: 10.1038/s41467-018-04572-3. PMID: 30013058;
PMCID: PMC6048166.

Yang Y, Mamouni K, Li X, Chen Y, Kavuri S, Du Y, Fu H, Kucuk O, Wu D. Repositioning Dopamine D2 Receptor Agonist Bromocriptine to Enhance Docetaxel Chemotherapy and Treat Bone Metastatic Prostate Cancer. Mol Cancer Ther. 2018 Sep;17(9):1859-1870. doi: 10.1158/1535-7163.MCT-17-1176. Epub 2018 Jun 15. PMID: 29907594; PMCID: PMC6125160.

Sime W, Niu Q, Abassi Y, Masoumi KC, Zarrizi R, Køhler JB, Kjellström S, Lasorsa VA, Capasso M, Fu H, Massoumi R. BAP1 induces cell death via interaction with 14-3-3 in neuroblastoma. Cell Death Dis. 2018 May 1;9(5):458. doi: 10.1038/s41419-018-0500-6. PMID: 29686263; PMCID: PMC5913307.

Xiong J, Pecchi VG, Qui M, Ivanov AA, Mo X, Niu Q, Chen X, Fu H, Du Y. Development of a Time-Resolved Fluorescence Resonance Energy Transfer Ultrahigh-Throughput Screening Assay for Targeting the NSD3 and MYC Interaction. Assay Drug Dev Technol. 2018 Feb/Mar;16(2):96-106. doi: 10.1089/adt.2017.835. PMID: 29634317; PMCID: PMC5865254.

Huard DJE, Crowley VM, Du Y, Cordova RA, Sun Z, Tomlin MO, Dickey CA, Koren J 3rd, Blair L, Fu H, Blagg BSJ, Lieberman RL. Trifunctional High-Throughput Screen Identifies Promising Scaffold To Inhibit Grp94 and Treat Myocilin-Associated Glaucoma. ACS Chem Biol. 2018 Apr 20;13(4):933-941. doi: 10.1021/acschembio.7b01083. Epub 2018 Feb 20. PMID: 29402077; PMCID: PMC6195314.

Boettcher M, Tian R, Blau JA, Markegard E, Wagner RT, Wu D, Mo X, Biton A, Zaitlen N, Fu H, McCormick F, Kampmann M, McManus MT. Dual gene activation and knockout screen reveals directional dependencies in genetic networks. Nat Biotechnol. 2018 Feb;36(2):170-178. doi: 10.1038/nbt.4062. Epub 2018 Jan 15.
PMID: 29334369; PMCID: PMC6072461.

Rusnak L, Fu H. Regulation of ASK1 signaling by scaffold and adaptor proteins. Adv Biol Regul. 2017 Dec;66:23-30. doi: 10.1016/j.jbior.2017.10.003. Epub 2017 Oct 16. PMID: 29102394.

Qi Q, Obianyo O, Du Y, Fu H, Li S, Ye K. Blockade of Asparagine Endopeptidase Inhibits Cancer Metastasis. J Med Chem. 2017 Sep 14;60(17):7244-7255. doi: 10.1021/acs.jmedchem.7b00228. Epub 2017 Aug 18. PMID: 28820254; PMCID: PMC5871875.

Giroud C, Du Y, Marin M, Min Q, Jui NT, Fu H, Melikyan GB. Screening and Functional Profiling of Small-Molecule HIV-1 Entry and Fusion Inhibitors. Assay Drug Dev Technol. 2017 Feb/Mar;15(2):53-63. doi: 10.1089/adt.2017.777. PMID: 28322598; PMCID: PMC5367911.

Umstead M, Xiong J, Qi Q, Du Y, Fu H. Aurora kinase A interacts with H-Ras and potentiates Ras-MAPK signaling. Oncotarget. 2017 Apr 25;8(17):28359-28372. doi: 10.18632/oncotarget.15049. PMID: 28177880; PMCID: PMC5438655.

Ivanov AA, Revennaugh B, Rusnak L, Gonzalez-Pecchi V, Mo X, Johns MA, Du Y, Cooper LAD, Moreno CS, Khuri FR, Fu H. (2017) The OncoPPi Portal: an integrative resource to explore and prioritize protein-protein interactions for cancer target discovery. Bioinformatics. 2017. November 24. doi: 10.1093/bioinformatics/btx743

Ivanov AA, Gonzalez-Pecchi V, Khuri LF, Niu Q, Wang Y, Xu Y, Bai Y, Mo X, Prochownik EV, Johns MA, Du Y, Khuri FR, Fu H. (2017) OncoPPi-informed discovery of mitogen-activated protein kinase kinase 3 as a novel binding partner of c-Myc. Oncogene. 2017. June 19. doi: 10.1038/onc.2017.180

Qi Q, Kang SS, Zhang S, Pham C, Fu H, Brat DJ, Ye K. (2017) Co-amplification of phosphoinositide 3-kinase enhancer A and cyclin-dependent kinase 4 triggers glioblastoma progression. Oncogene. 2017 Apr 3. doi: 10.1038/onc.2017.67. [Epub ahead of print] PMID:28368413

Zhang Z, Obianyo O, Dall E, Du Y, Fu H, Liu X, Kang SS, Song M, Yu SP, Cabrele C, Schubert M, Li X, Wang JZ, Brandstetter H, Ye K. (2017) Inhibition of delta-secretase improves cognitive functions in mouse models of Alzheimer's disease. Nat Commun. 2017 Mar 27;8:14740. doi: 10.1038/ncomms14740. PMID:28345579

Li Z*, Ivanov AA*, Su R*, Gonzalez-Pecchi V, Qi Q, Liu S, Webber P, McMillan E, Rusnak L, Pham C, Chen X, Mo X, Revennaugh B, Zhou W, Marcus AI, Harati S, Chen X, Johns MA, White MA, Moreno C, Cooper LAD, Du Y, Khuri FR, Fu H (2017). The OncoPPi network of cancer-focused protein-protein interactions to inform biological insights and therapeutic strategies. Nature Communications 2017 8, 14356 doi: 10.1038/ncomms14356. PMID: 28205554 (*co-first author)

Mo XL, Qi Q, Ivanov AA, Niu Q, Luo Y, Havel J, Goetze R, Bell S, Moreno CS, Cooper LA, Johns MA, Khuri FR, Du Y, Fu H. (2017) AKT1, LKB1, and YAP1 revealed as MYC interactors with NanoLuc-based protein-fragment complementation assay. Mol Pharmacol. 2017 Apr;91(4):339-347. doi: 10.1124/mol.116.107623. PMID: 28087810

Harati S, Cooper LAD, Moran JD, Giuste F, Du Y, Ivanov AA, Johns MA, Khuri FR, Fu H, Moreno CS, (2017) MEDICI: Mining Essentiality Data to Identify Critical Interactions for Cancer Drug Target Discovery and Development, PLoS One. 2017 Jan 24;12(1):e0170339.. doi: 10.1371/journal.pone.0170339.

Horton JR, Liu X, Gale M, Wu L, Shanks JR, Zhang X, Webber PJ, Bell JS, Kales SC, Mott BT, Rai G, Jansen DJ, Henderson MJ, Urban DJ, Hall MD, Simeonov A, Maloney DJ, Johns MA, Fu H*, Jadhav A*, Vertino PM, Yan Q, Cheng X. (2016) Structural Basis for KDM5A Histone Lysine Demethylase Inhibition by Diverse Compounds. Cell Chem Biol. 2016 Jul 21;23(7):769-81. doi: 10.1016/j.chembiol.2016.06.006. PMID: 27427228(*co-senior author)

Gu L, Zhang H, Liu T, Zhou S, Du Y, Xiong J, Yi S, Qu CK, Fu H, Zhou M. (2016) Discovery of Dual Inhibitors of MDM2 and XIAP for Cancer Treatment. Cancer Cell. 2016 Oct 10;30(4):623-636. doi: 10.1016/j.ccell.2016.08.015. PMID: 27666947

Gerhard DS, Clemons PA, Shamji AF, Hon C, Wagner BK, Schreiber SL, Krasnitz A, Sordella R, Sander C, Lowe SW, Powers S, Smith K, Aburi M, Iavarone A, Lasorella A, Silva J, Stockwell BR, Califano A, Boehm JS, Vazquez F, Weir BA, Hahn WC, Khuri FR, Moreno CS, Johns M, Fu H, Nikolova O, Mendez E, Gadi VK, Margolin AA, Grandori C, Kemp CJ, Warren EH, Riddell SR, McIntosh MW, Gevaert O, Kuo CJ, Ji HP, Dhruv H, Finlay D, Kiefer J, Kim S, Vuori K, Berens ME, Hangauer M, Boettcher M, Weissman JS, Bivona TG, Bandyopadhyay S, McManus MT, McCormick F, Aksoy O, Simonds EF, Zheng T, Chen J, An Z, Balmain A, Weiss WA, Chen K, Liang H, Scott KL, Mills GB, Posner BA, MacMillan J, Minna J, White M, Roth MG, Jagu S, Mazerik J. (2016) Transforming Big Data into cancer-relevant insight: An initial, multi-tier approach to assess reproducibility and relevance, Molecular Cancer Res. 2016 Aug;14(8):675-82. DOI: 10.1158/1541-7786.MCR-16-0090. PMID: 27401613

Mo XL, Fu H. (2016) BRET: NanoLuc-Based Bioluminescence Resonance Energy Transfer Platform to Monitor Protein-Protein Interactions in Live Cells. Methods Mol Biol. 2016;1439:263-71. doi: 10.1007/978-1-4939-3673-1_17. PMID: 27317001

Horton JR, Engstrom A, Zoeller EL, Liu X, Shanks JR, Zhang X, Johns MA, Vertino PM, Fu H, Cheng X. (2015) Characterization of a linked Jumonji domain of the KDM5/JARID1 family of histone H3 lysine 4 demethylases. J Biol Chem. 2015 Dec 8. pii: jbc.M115.698449. PMID: 26645689

Mo XL, Luo Y, Ivanov AA, Su R, Havel JJ, Li Z, Khuri F, Du Y, Fu H. (2015) Enabling systematic interrogation of protein-protein interactions in live cells with a versatile ultra-high-throughput biosensor platform. J Mol Cell Biol. 2016 Jun;8(3):271-81.; doi: 10.1093/jmcb/mjv064 PMID: 26578655

Marin M, Du Y, Giroud C, Kim JH, Qui M, Fu H, Melikyan GB. (2015) High-Throughput HIV–Cell Fusion Assay for Discovery of Virus Entry Inhibitors. Assay Drug Dev Technol. 2015 Apr;13(3):155-66. doi:10.1089/adt.2015.639. PMID: 25871547

Gu L, Zhang H, Liu T, Zhou S, Du Y, Xiong J, Yi S, Qu CK, Fu H, Zhou M.(2016) Discovery of Dual Inhibitors of MDM2 and XIAP for Cancer Treatment.  Cancer Cell. 2016 Oct 10;30(4):623-636. doi: 10.1016/j.ccell.2016.08.015. PMID: 27666947

Horton JR, Liu X, Gale M, Wu L, Shanks JR, Zhang X, Webber PJ, Bell JS, Kales SC, Mott BT, Rai G, Jansen DJ, Henderson MJ, Urban DJ, Hall MD, Simeonov A, Maloney DJ, Johns MA, Fu H, Jadhav A, Vertino PM, Yan Q, Cheng X. (2016)  Structural Basis for KDM5A Histone Lysine Demethylase Inhibition by Diverse Compounds. Cell Chem Biol. 2016 Jul 21;23(7):769-81. doi: 10.1016/j.chembiol.2016.06.006. PMID: 27427228

Webber PJ, Park C, Qui M, Ramalingam SS, Khuri FR, Fu H, Du Y (2015) Combination of heat shock protein 90 and focal adhesion kinase inhibitors synergistically inhibits the growth of non-small cell lung cancer cells. Oncoscience. 2015 Sep 15; 2(9):765-76. PMID: 26501082